USPTO Patents Application 09939518

01/06/2005.10:11 .FAX 7275078668

Smith&Hopen , P. A.

0005/009

AMENDMENT TO THE CLAIMS:

1. (Previously Presented) A method for facilitating the delivery of a desired molecule
into a target tissue consisting essentially of the steps of:

introducing a molecule into a target tissue comprising a cell;

applying an electric field to the target tissue, the application of the electric field
consisting of a single continuous low-level electric field applied for a duration
of 100ms to 20 minutes; and

effecting a change in porosity of the cell of the target tissue in response to the
application of the electric field, the change in porosity sufficient to facilitate
entry of a desired molecule into an interior of the cell.

2. (Previously Presented) The method recited in Claim 1, wherein the duration
of the applying step is in a range of 100ms to 100 sec.

3. (Cancelled)

4. (Original) The method recited in Claim 1, wherein the low-level electric
field has a field strength comprising 200V/cm or less.

5. (Cancelled)

6. (Original) The method recited in Claim 1, wherein the electric field
comprises a pulse selected from a group of waveforms consisting of square,
rectangular, exponentially decaying, exponentially increasing, bipolar, and
sinusoidal; waveforms having a nongeometrically characterizable shape;
waveforms characterizable by a mathematical function; waveforms
characterizable by a mathematical approximation; waveforms with at least one
of an AC or a DC offset signal; and waveforms without an AC or a DC offset
signal*

7. (Cancelled)

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01/06/2005.10:1 1 .FAX 7275078668

Smit h&Hopen , P . A.

0 006/009

8. (Original) The method recited in Claim 1, wherein the introducing step
comprises the step selected from a group consisting of syringe injection, jet
injection, oral dosing, transdermal delivery, infusion into tissue, and infusion
into a blood vessel.

9. (Cancelled)

10. (Original) The method recited in Claim 1, wherein the target tissue is
selected from a group consisting of skin, tumor, muscle, blood, blood vessel,
brain, lymph, liver, pancreas, bone, colon, cardiac, lung, breast, testes, cornea,
prostate, and intestine.

Claims 1 1-20 (Cancelled)

21. (Previously Presented) A method for facilitating the delivery of a desired
molecule into a target tissue comprising the steps of:

introducing a molecule into a target tissue comprising a cell; and

applying a continuous low-level electric field to the target tissue for a
duration of 200ms to 20 minutes to effect a change in porosity of the cell of the
target tissue sufficient to facilitate entry of a desired molecule into an interior
of the cell.

22. (Previously Presented) The method recited in Claim 21, wherein the
duration of the applying step is in a range of 200ms to 100 sec.

23. (Previously Presented) The method recited in Claim 21, wherein the low-
level electric field has a field strength comprising 200V/cm or less.

24. (Previously Presented) The method recited in Claim 21, wherein the
applying step comprises applying a plurality of substantially continuous low-
level electric pulses to the target tissue, wherein the duration of each
substantially continuous low-level electric field is sufficient to effect a change

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'01/06/2005 .10:1 1 FAX 7275078668

Smith&Hopen, P . A .

$007/009

is porosity of the cell of the target tissue sufficient to facilitate entry of a
desired molecule into an interior of the cell

25. (Previously Presented) The method recited in Claim 21 3 wherein the
electric field comprises a pulse selected from a group of waveforms consisting
of square, rectangular, exponentially decaying, exponentially increasing,
bipolar, and sinusoidal; waveforms having a nongeometrically characterizable
shape; waveforms characterizable by a mathematical function; waveforms
characterizable by a mathematical approximation; waveforms with at least one
of an AC or a DC offset signal; and waveforms without an AC or a DC offset
signal.

26. (Previously Presented) The method recited in Claim 25, wherein the
electric field comprises a pulse comprising a combination of at least two of the
pulses selected from the group of waveforms.

27. (Previously Presented) The method recited in Claim 21, wherein the
introducing step comprises the step selected from a group consisting of syringe
injection, jet injection, oral dosing, transdermal delivery, infusion into tissue,
and infusion into a blood vessel.

28. (Previously Presented) The method recited in Claim 21, wherein the target
tissue is selected from a group consisting of skin, tumor, muscle, blood, blood
vessel, brain, lymph, liver, pancreas, . bone, colon, cardiac, lung, breast, testes,
cornea, prostate, and intestine.

Claims 29-52 (Canceled)

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